Intriguingly, the two GFP-positive cell populations remain distinctly separated during aging, presenting a novel technique for identifying and isolating cell populations enriched for either ISCs or EBs at any time point during aging. Check if you have access through your login credentials or your institution. However, this only holds true for a young and healthy gut. Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. Also, in the plot shown here, the limit for autofluorescence was not adjusted, hence cells that plot above 10 2 on the logarithmic y-axis are actually autofluorescent and could be mistaken as GFP-positive cells. Nature , e
Drosophila has long been an excellent model organism for studying stem cell biology. E The histogram plot shows the number of cells and their GFP intensity. The authors declare that they have no competing financial interests. Export Citation Download to citation manager. Navigate This Article Top Abstract Results Discussion Materials and Methods Acknowledgments Footnotes References. Acknowledgments We are grateful to Gabriele Allies for excellent technical assistance.
The adult Drosophila posterior midgut is maintained by pluripotent stem cells — Johns Hopkins University
Metabolic regulation of stem cell behavior and implications for aging. Schroder C Deegener P in Handbuch der Entomologie , The intestinal tract and its appendages Translated from German ed Schroder C Fischer , Jena, Germany , Vol 1 , pp — Make sure that the fluidic system is free of air bubbles. Molecular and cellular organization of the adult Drosophila middle midgut.
The adult Drosophila posterior midgut is maintained by pluripotent stem cells
Description: A site license includes a minimum of four years of archived content ; institutions can add additional archived content to their license at any time. Link to citation list in Scopus. More Dl expression could be detected in cells present in gate P5 vs. JNK Activity in Somatic Stem Cells Causes Loss of Tissue Homeostasis in the Aging Drosophila Gut.